Active antibacterial coating of cotton fabrics with antimicrobial proteins

Abstract The prevention of bacteria colonization by immobilizing proteins with antimicrobial activity onto cotton fabrics was investigated. Such coatings have potential applications in medical dressing materials used wound care and healing. Two lysozyme hydramacin-1 (HM-1) were surface immobilized through two linkers (3-aminopropyl) triethoxysilane (APTES) citric acid the presence water soluble carbodiimide coupling reagent 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate. Surface composition analysis attenuated total reflection-Fourier transform infrared X-ray photoelectron spectroscopies confirmed formation protein-cellulose conjugates. Antimicrobial activities different functionalized surfaces found to vary between APTES directed coatings. Citric treated samples demonstrated superior against Gram-positive Bacillus subtilis , whereas HM-1 an advantage inhibiting growth Gram-negative Escherichia coli . antibacterial stability protein following sonication, boiling chemical treatment noticeably higher than that corresponding fabrics. dual coating fibers both afforded efficient bacterial species. results suggest peptides represents a feasible approach for developing active prohibit strains can be potentially cotton-based